|
KMID : 0894520030070020095
|
|
Development & Reproduction
2003 Volume.7 No. 2 p.95 ~ p.103
|
|
|
Effect of Ruthenium Red and Ryanodine on Calcium Ion Metabolism in Oocyte and Early Embryo of Mouse
|
|
이준영/Lee JY
홍순갑/김태식/민병열/김해권/윤용달/Hong SG/Kim TS/Min BY/Kim HK/Yoon YD
|
|
|
Abstract
|
|
|
|
Intracellular calcium is an important physiological factor in most cells, and ruthenium red and ryanodine play an important role as calcium modulators. Ruthenium red inhibits calcium-induced calcium release(CICR) from the intracellular calcium store. Ryanodine activates calcium release through ryanodine channel. The present experiment was performed to investigate the effects of two modulators on calcium ion metabolism and to determine their dose-dependency in oocyte and early embryo of mouse. Intracellular calcium ion concentration was measured in realtime by using confocal laser scanning microscope(CLSM) after loading of Fluo-3/AM in mouse oocytes and early embryos. Ruthenium red decreased intracellular calcium ion concentration in oocytes and early embryos at its high concentration(30, 300 μM). Ryanodine increased intracellular calcium ion concentration in oocytes and early embryos in low concentration(0.01 μM) but decreased that at higher concentrations(1, 10 μM). These results indicate that two modulators affected calcium ion metabolism in oocyte and early embryo of mouse, and their dose-dependency was different from somatic cell including myocytes.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|